Journal Bhavnagar University

current issue

 

Jun, 2016
Vol-6 Issue - 1 (Semi-annual Publication)

Title of the article : Determination of Sex by Dental Pulp Tissue - A Single Blind Study

Authors : MAHARUDRAPPA BASNAKER , CHANCHAL MOOLRAJANI

Abstract

Background : Determination of sex in cases of natural disaster, military conflict, terrorism and mass transportation accidents becomes essential to identify the deceased person. Various tools are used to ascertain the sex determination in forensic medicine. Aim of the study: In the present study, we intend to evaluate the presence of Barr body in the histologically processed human dental pulp tissue taken from the freshly extracted teeth.

Study design : It was a single blind in-vitro study consisting of 40 healthy teeth extracted from both male and female subjects in the age range of 18-45yrs. The samples were processed and evaluated by the principal investigator and the results were correlated with that of the evaluator.

Materials and methods:The freshly extracted teeth stored in 10 % formalin were split and pulp tissue was taken out, processed and subjected to acridine orange staining as described by Von Bertalanfy et al. Stained sections were examined under fluorescent microscope and the percentage of Barr body positive cells was determined. Results: The data was statistically evaluated using chi-square test, the sensitivity came out to be 96% and specificity as 87%. The percentage of Barr body positive cells in male samples ranged from 0-2% while in female sample ranged from 19 to 48%.

Conclusion : The presence of Barr bodies in dental pulp tissue proved to be a useful tool in the determination of sex in forensic odontology. This study can further be extended in the evaluation of human identification in natural calamities and mass disasters, when the body is mutilated beyond the scope of identification.

Key-words : Forensic odontology, Barr bodies, human dental pulp, acridine orange, fluorescent microscopy.



ISSN (ONLINE) - 2321 - 7553

ISSN (PRINT) - 2249-443x

 

 


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Determination of Sex by Dental Pulp Tissue - A Single Blind Study
AUTHORS: MAHARUDRAPPA BASNAKER , CHANCHAL MOOLRAJANI
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